Rapid Semiquantitative Method for Screening Large Numbers of Virus Samples by Negative Staining Electron Microscopy

Rapid semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy.

A rapid, semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy is presented. Results obtained by this method are compared with results obtained by the pseudoreplica method and a measureddrop method. A figure is presented which represents the limit of detectability for virus by negative staining.

An Improved Staining Method for Electron Microscopy

A n a q u e o u s so lu t ion of u r a n y l ace t a t e was first e m p l o y e d as a n e l ec t ron s ta in by W a t s o n (1958). W e h a v e f o u n d t h a t this s ta in , a l t h o u g h f r e q u e n t l y effective, is capr ic ious . T h e s ta in m a y no t inc rease con t r a s t of a g iven spec imen , a n d m a y , in o the r ins tances , l eave u n d e s i r a b l e c o n t a m i...

Electron Microscopy of Fowl Pox Virus

Optimized negative-staining electron microscopy for lipoprotein studies.

BACKGROUND Negative-staining (NS), a rapid, simple and conventional technique of electron microscopy (EM), has been commonly used to initially study the morphology and structure of proteins for half a century. Certain NS protocols however can cause artifacts, especially for structurally flexible or lipid-related proteins, such as lipoproteins. Lipoproteins were often observed in the form of rou...

Large-volume en-bloc staining for electron microscopy-based connectomics

Large-scale connectomics requires dense staining of neuronal tissue blocks for electron microscopy (EM). Here we report a large-volume dense en-bloc EM staining protocol that overcomes the staining gradients, which so far substantially limited the reconstructable volumes in three-dimensional (3D) EM. Our protocol provides densely reconstructable tissue blocks from mouse neocortex sized at least...